The Study of Human CD4+ T-cells Using Class II Tetramers
نویسندگان
چکیده
Esta revisión habla sobre la emergencia de los tetrámeros como reactivos para el estudio de la inmunidad humana. Los tetrámeros del complejo mayor de histocompatibilidad (MHC) han generado nuevas e interesantes posibilidades para la caracterización de las células T específicas de antígeno. Estos tetrámeros tienen la capacidad única de marcar y subsecuentemente facilitar el aislamiento o la estimulación de células T con una sola restricción de péptido-MHC. Por eso los tetrámeros tienen el potencial de refinar o incluso redefinir las respuestas inmunitarias T tal y como las entendemos actualmente. Además de una revisión breve sobre la historia y el desarrollo de los tetrámeros, este artículo ofrece un resumen de la producción de tetrámeros de clase II usando un sistema de expresión en Drosophila, y apunta varios métodos para su aplicación. Las similitudes y diferencias más importantes entre los tetrámeros de clase II, técnicas tradicionales y otras técnicas alternativas también serán detalladas, junto con una previsión de las direcciones futuras de esta tecnología.
منابع مشابه
MHC Class II Tetramers Made from Isolated Recombinant α and β Chains Refolded with Affinity-Tagged Peptides
Targeting CD4+ T cells through their unique antigen-specific, MHC class II-restricted T cell receptor makes MHC class II tetramers an attractive strategy to identify, validate and manipulate these cells at the single cell level. Currently, generating class II tetramers is a specialized undertaking effectively limiting their use and emphasizing the need for improved methods of production. Using ...
متن کاملUltrasensitive detection and phenotyping of CD4+ T cells with optimized HLA class II tetramer staining.
HLA class I tetramers have revolutionized the study of Ag-specific CD8+ T cell responses. Technical problems and the rarity of Ag-specific CD4+ Th cells have not allowed the potential of HLA class II tetramers to be fully realized. Here, we optimize HLA class II tetramer staining methods through the use of a comprehensive panel of HIV-, influenza-, CMV-, and tetanus toxoid-specific tetramers. W...
متن کاملUse of HLA class II tetramers in tracking antigen-specific T cells and mapping T-cell epitopes.
The highly specific interaction between major histocompatibility complex (MHC)/peptide and its cognate T-cell receptor (TCR) directs to the specificity of the T-cell response. Although the interaction affinity of individual MHC/peptide and TCR molecules is low, multimerization of the MHC/peptide complexes increases the avidity of their interaction with TCRs, and enables the complexes to be used...
متن کاملMultiplex mapping of CD4 T cell epitopes using class II tetramers.
With the advent of class II tetramer technology, a tetramer-guided epitope mapping (TGEM) technique was developed for the identification of CD4+ T cell epitopes. This allowed the direct identification of epitopes recognized by the responding T cells, which were restricted to the single MHC allele of interest. However, as each individual carries multiple class II alleles, it would be advantageou...
متن کاملDetection of GAD65-specific T-cells by major histocompatibility complex class II tetramers in type 1 diabetic patients and at-risk subjects.
Soluble HLA-DR401 or -DR404 tetramers containing a peptide corresponding to an immunodominant epitope from human GAD65 were used to analyze peripheral blood T-cells of newly diagnosed type 1 diabetic patients and at-risk subjects. Peripheral blood mononuclear cells were expanded on antigen-presenting cells presenting GAD65 peptide and subsequently activated with specific plate-bound class II-pe...
متن کاملMHC class II/ESO tetramer-based generation of in vitro primed anti-tumor T-helper lines for adoptive cell therapy of cancer.
Generation of tumor-antigen specific CD4(+) T-helper (T(H)) lines through in vitro priming is of interest for adoptive cell therapy of cancer, but the development of this approach has been limited by the lack of appropriate tools to identify and isolate low frequency tumor antigen-specific CD4(+) T cells. Here, we have used recently developed MHC class II/peptide tetramers incorporating an immu...
متن کامل